![]() IFAT and ELISA are amongst the most common serological techniques used for the diagnosis and for clinical and research studies on canine and feline L. infantum are increasingly being reported in Europe. Moreover, clinical cases of feline leishmaniosis and subclinical infections due to L. infantum is strongly suspected as infected cats are able to transmit the parasite to vector sand flies. Dogs are the primary reservoir host however other animal species can be infected, including cats. Leishmaniosis due to Leishmania infantum is a zoonotic vector-borne disease of worldwide importance, transmitted by phlebotominae sand flies. The overall performance of all serological techniques was good and the most accurate test for anti- Leishmania antibody detection in feline serum samples was WB. IFAT was more sensitive than ELISA (75 vs 70%) for the detection of subclinical infection while ELISA was better for diagnosing clinical leishmaniosis when compared with IFAT (98 vs 97%). The median seroprevalence in the sample used for evaluating the performance of tests was estimated at 0.27. The latent data and observations from the joint posterior were simulated in the Bayesian approach by an iterative Markov Chain Monte Carlo technique using the Gibbs sampler for estimating sensitivity and specificity of the three tests. Statistical analysis was performed through a written routine with MATLAB software in the Bayesian framework. WB was considered positive in presence of at least a 18 KDa band. MethodsĬut-offs were set at 80 titre for IFAT and 40 ELISA units for ELISA. infantum antibodies in feline serum samples obtained from endemic ( n = 76) and non-endemic ( n = 64) areas and from cats affected by feline leishmaniosis ( n = 21) by a Bayesian approach without a gold standard. The aim of the present study was to assess diagnostic performance of IFAT, ELISA and WB to detect anti- L. The immunofluorescent antibody test (IFAT), the enzyme-linked immunosorbent assay (ELISA) and western blot (WB) are the serological tests more frequently used. Anti- Leishmania antibodies are increasingly investigated in cats for epidemiological studies or for the diagnosis of clinical feline leishmaniosis.
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